Voltage-sensitive nitrendipine binding in an isolated cardiac sarcolemma preparation.
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چکیده
منابع مشابه
Effects of bepridil and diltiazem on [3H]nitrendipine binding to canine cardiac sarcolemma. Potentiation of pharmacological effects of nitrendipine by bepridil.
[3H]Nitrendipine binds to canine cardiac sarcolemma in a specific, saturable and rapid manner. Bepridil, a Ca++ channel inhibitor, stimulates this binding at submicromolar concentrations, but inhibits it noncompetitively at higher concentrations (IC50 = 15.8 microM). The increase in binding was due primarily to a 30% increase in the association rate constant (k+1) of [3H]nitrendipine, causing a...
متن کاملCharacterization of endogenous phosphorylation in isolated cardiac sarcolemma.
The cardiac sarcolemma contains kinases which catalyze the incorporation of 32P-phosphate into acid stable and acid precipitable membrane components of low molecular weight. The phosphorylation is not influenced by cyclic AMP or calmodulin. Analysis of phosphorylation products using proteolytic digestion, organic solvent extraction, thin layer chromatography and gel filtration reveals both poly...
متن کاملEffects of Bay k 8644, a dihydropyridine analog, on [3H]nitrendipine binding to canine cardiac sarcolemma and the relationship to a positive inotropic effect.
Equilibrium dissociation constants of Bay k 8644, a calcium agonist, and nitrendipine, a calcium antagonist, were determined in canine cardiac sarcolemma. The equilibrium dissociation constant for Bay k 8644 was compared to the concentration that produced a fifty percent increase, and the equilibrium dissociation constant for nitrendipine was compared to the concentration that produced a fifty ...
متن کاملEffects of Bay k 8644, a Dihydropyridine Analog, on [H]Nitrendipine Binding to Canine Cardiac Sarcolemma and the Relationship to a Positive Inotropic Effect
Equilibrium dissociation constants of Bay k 8644, a calcium agonist, and nitrendipine, a calcium antagonist, were determined in canine cardiac sarcolemma. The equilibrium dissociation constant for Bay k 8644 was compared to the concentration that produced a fifty percent increase, and the equilibrium dissociation constant for nitrendipine was compared to the concentration that produced a fifty ...
متن کاملGlobal ischemia increases the density of voltage-dependent calcium channels in porcine cardiac sarcolemma.
The objective of this work was to determine whether normothermic global cardiac ischemia in a porcine model was associated with a change in the density (Bmax) of voltage-dependent calcium channels in myocardial sarcolemmal membranes. Pigs were anesthetized, a thoracotomy was performed, and samples were taken of the left and right ventricles from control and ischemic hearts. Dihydropyridine-bind...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)35849-0